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High myopia is a state of refractive error with myopia over -6.00D. High myopia is typically accompanied by multiple fundus lesions, thus making patients with high myopia suffer from varying degrees of impairment in visual function. As an emerging auxiliary way in ophthalmology, optical coherence tomography angiography(OCTA)can efficiently and non-invasively obtain microvascular stratified images of the retina and choroid and quantitatively analyze blood flow signals. Since the advent of OCTA, there have been numerous studies observing fundus changes in those with high myopia through OCTA. In this paper, some studies in which OCTA is applied to obtain retinal and choroidal thickness from patients with high myopia are reviewed, with a view to revealing the correlation between high myopia and the parameters such as retinal thickness, choroidal thickness, vessel density and the area of the foveal avascular zone and providing novel ideas to deeply investigate the mechanism of high myopia and delay the occurrence and development of high myopia.
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Xiao Xumingtang in The Catalogue of Famous Ancient Classics （The First Batch） issued by the National Administration of Traditional Chinese Medicine is derived from the Important Prescriptions Worth a Thousand Gold for Emergency （Bei Ji Qian Jin Yao Fang） written by SUN Si-miao in the Tang dynasty. The present study systematically explored the origin， development， historical evolution， and clinical application of Xiao Xumingtang. As revealed by the results， Xiao Xumingtang as well as its analogues are primary prescriptions indicated for apoplexy before the Tang and Song dynasties and serve as the benchmark for the treatment of apoplexy. After the Song dynasty， due to the changes in the understanding of the pathogenesis of apoplexy and the limitations of the understanding of Xiao Xumingtang， its clinical application to apoplexy gradually decreased. In modern times， it has been re-recognized and applied， during which its clinical applications have undergone great changes. Its clinical applications are extensive， involving a variety of diseases related to the brain and nervous systems， such as stroke and its sequelae， peripheral facial paralysis， rheumatoid arthritis， hypertension， and other diseases related to the motor nervous system. Its primary indications are stroke and its sequelae， followed by peripheral facial paralysis. Other new indications are gradually found. This study is expected to provide references for the clinical application of Xiao Xumingtang and the transformation of new drugs.
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Objectives@#This study evaluated dietary behavior and nutritional status according to the metabolic syndrome status in Korean menopausal women. @*Methods@#The subjects were 1,392 menopausal women aged 50 to 64 who took part in the Korea National Health and Nutrition Examination Survey of 2016 and 2017.Subjects were classified into normal (NOR) group, pre-metabolic syndrome (Pre-MetS) group, and metabolic syndrome (MetS) groups according to the number of metabolic syndrome risk factors present. @*Results@#The overall prevalence of metabolic syndrome was 33.7%. Using the NOR group as a reference, the odds of belonging to the MetS group in Model 1 adjusted for age were higher at 53% (OR = 1.53, 95% CI:1.011-2.307) for ‘not used’ subjects compared to ‘used’ subjects of the nutrition labeling system. Using the NOR group as a reference, every 1g increase in the intake of monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA) decreased the odds of belonging to the MetS group in Model 1 adjusted for age by 3% (MUFA, OR = 0.97, 95% CI:0.946-0.991; PUFA, OR = 0.97, 95% CI:0.942-0.993). @*Conclusions@#These results suggest that to reduce the number of risk factors of metabolic syndrome in menopausal women, nutritional education should emphasize the adequate intake of riboflavin, unsaturated fatty acids, protein, and calcium, and also encourage the recognition and use of nutritional labeling. Results of this study are expected to be utilized as basic data for the health management of menopausal women.
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Objective@#To explore the clinical effect of Guben-Huoxue decoction on chronic obstructive pulmonary disease (COPD) and its influence on patients'respiratory rehabilitation and quality of life.@*Methods@#A total 108 patients with COPD admitted to our hospital from May 2013 to January 2018 were selected as the research subjects. The patients were divided into control group and observation group by random number table grouping method, 54 in each group. All patients were given basic treatment such as oxygen inhalation, anti-infection, bronchiectasis, and correction of acidosis. The control group was treated with budesonide formoterol powder inhalation, while the observation group was treated with Guben-Huoxue decoction on the basis of the control group. The maximum ventilation volume per minute (MVV), forced vital capacity (FVC), forced expiratory volume in the first second (FEV1), forced expiratory volume in the second (FEV1%) were measured by pulmonary function meter. Adverse reactions were recorded.@*Results@#The difference of FVC, MVV, FEV1%, symptoms, activity ability, disease influence and comprehensive scores in the observation group were significantly greater than those in the control group (t values were 6.480, 13.679, 5.853, 3.743, 5.786, 5.309, 5.058, respectively, all Ps<0.05). The total effective rate (94.4%) in the observation group were significantly higher than those in the control group (79.6%); the total incidence of adverse reactions (9.3%) was significantly lower than that of the control group (25.9%), and the difference was statistically significant (P<0.05).@*Conclusions@#The Guben-Huoxue decoction can improve the clinical symptoms and pulmonary function of COPD patients, promote respiratory rehabilitation and improve the quality of life.
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Objective To explore the clinical effect of Guben-Huoxue decoction on chronic obstructive pulmonary disease (COPD) and its influence on patients'respiratory rehabilitation and quality of life. Methods A total 108 patients with COPD admitted to our hospital from May 2013 to January 2018 were selected as the research subjects. The patients were divided into control group and observation group by random number table grouping method, 54 in each group. All patients were given basic treatment such as oxygen inhalation, anti-infection, bronchiectasis, and correction of acidosis. The control group was treated with budesonide formoterol powder inhalation, while the observation group was treated with Guben-Huoxue decoction on the basis of the control group. The maximum ventilation volume per minute (MVV), forced vital capacity (FVC), forced expiratory volume in the first second (FEV1), forced expiratory volume in the second (FEV1%) were measured by pulmonary function meter. Adverse reactions were recorded. Results The difference of FVC, MVV, FEV1%, symptoms, activity ability, disease influence and comprehensive scores in the observation group were significantly greater than those in the control group (t values were 6.480, 13.679, 5.853, 3.743, 5.786, 5.309, 5.058, respectively, all Ps<0.05). The total effective rate (94.4%) in the observation group were significantly higher than those in the control group (79.6%); the total incidence of adverse reactions (9.3%) was significantly lower than that of the control group (25.9%), and the difference was statistically significant (P<0.05). Conclusions The Guben-Huoxue decoction can improve the clinical symptoms and pulmonary function of COPD patients, promote respiratory rehabilitation and improve the quality of life.
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AIM:To study the effect of ClC-3 gene over-expression on thyroid structure and function in mice. METHODS:Three-months-old FVB mice were used to study the difference of thyroid structure and function between wild-type(WT)mouse and ClC-3 transgene mice.The expression and distribution of ClC-3 in the thyroid of mice were deter-mined by the methods of qPCR,Western blot and immunofluorescence.Behavioral monitoring was performed on the daily activities of mice.Serum concentrations of total triiodothyronine(TT3), total thyroxine(TT4)and thyrotropin(TSH) were measured by ELISA.RESULTS:Compared with the WT group,the expression of ClC-3 in the thyroid of ClC-3 trans-gene group was significantly increased(P<0.05).The thyroid gland showed obvious hyperplasia and the folliculi glandu-lae thyreoideae was significantly bigger in ClC-3 transgene mice(P<0.05).The weight loss was increased in ClC-3 trans-gene mice(P<0.05).The expression of TT3 and TT4 were significantly higher than that of WT group(P<0.05),but the change of TSH was not obvious.CONCLUSION:ClC-3 over-expression results in thyroid hyperplasia and thyroid hor-mone secretion.This study suggests that ClC-3 is likely to be involved in the synthesis of thyroid hormones.
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Object To develop an easy-assembled and-disassembled image diagnostic module to solve the problems of the tent-form field medical system in size, weight, mobility, accessories, protection and etc, which is of high references for combination and integration scheme of large-scale digital imaging equipment by adopting box instrumentation.Methods The module had its design executed with system modeling, structure simulation and CAD after its service requirements were analyzed. The design fell into the ones for overall framework, combination mode, packaging and buffer vibration isolation, ergonomics, box instrumentation, radiography accessories for vertical and horizontal positions, protection device and etc.Results The module was gifted with radiodiagnosis-related performances such as digital radiography, digital diagnosis,diagnosis reporting, compatibility to vertical- and horizontal- position radiography, and radiation protection during war conditions.Conclusion The module developed gains advantages in power, continuous radiography, assembling and disassembling, accessories and protection, and thus meets the requirements of the tent-form field medical system in mass casualties imaging diagnosis.
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Object To study on the X-ray protection module in the radiodiagnosis unit to protect X-ray technicians, other medical staffs and etc against radiation during standing and lying X-ray examinations in the tent-form field medical system.Methods A local tightness protection solution was put forward involving in lead protective materials, standing shoot rack,diagnosis couch as well as considerations on the service orientation of the tent-form field medical system and the operation mode of the X-ray tent. A protective support with fast-plug and lateral folding structure was designed based on X-ray radiation protection principle and lead equivalent calculation of the lead curtain. An X-ray protection module was developed with the lend curtain, protective support, warning sign and etc.Results The dose peaked at vertical direction, and at vertical direction the annual dose to the public 1 m away from the tent was 78.8μSv which was far lower than the limit value 1 mSv. The strength of the protective support met the requirements, and the maximum deformation of the support bar was 15 mm after the suspension of the lead curtain.Conclusion The X-ray protection module gains advantages in design, structure, operation,rapid deployment and withdrawal, handling and transport as well as protective effective according to GB 18871—2002 regulation, and thus can be used for X-ray diagnosis at peace and war time.
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Objective To develop a medical technical support module and box-type medical technical equipment so as to supply medical oxygen and water for the tent-form field medical system at field conditions.Methods The medical technical support module had its service orientation analyzed to determine its functional requirements and technical indexes, which involved in PSA oxygen generation technology with variable frequency,constant pressure and self-adaptability to altitudes as well as medical water preparation technology by integrated membrane separation. Box-type modules for medical oxygen generation and medial water preparation were trial-produced with the mode of integrated box and instruments as well as high-density polyethylene as the box material.Results The oxygen production was 1.3 Nm3/h,and the oxygen concentration was 92.7%.The flow volume of cleaning water and purified water was 433.7 and 37.2 L/h respectively at 25℃,and the water quality accorded with the relevant water quality indexes of GB 5749—2006 and the national pharmacopoeia(2010 edition). Conclusion This set of medical equipment can meet the design requirements and can effectively guarantee the medical oxygen and medical water needed for medical treatmentin field tent hospital under field conditions. [Chinese Medical Equipment Journal,2018,39(5):7-11]
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Objective To develop a set of field tent hospital system to further enhance campaign-level medical support service. Methods The system had its service orientation, functional requirements, component unit and modular elements determined with system analysis. The technical architecture was realized with tent combined with boxes, and the system modules were designed with the method of integrated box and instruments.Results The system had its design executed from the aspects of layout,composition of grid tent,medical functional units,support unit and packaging unit,which was applied in a series of military medical support operations in foreign countries and China as well as in the construction of national medical emergency rescue forces. Conclusion The system gains advantages in modularity, integration, completing, informatization and standardization,and thus greatly enhances the military medical support ability and national emergency medical rescue ability in China.[Chinese Medical Equipment Journal,2018,39(5):1-6,16]
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Objective To study the clinical effect of Periocline ointment as the auxiliary treatment of chronic periodontitis. Methods 100 cases of chronic periodontitis in our hospital were selected as the subjects (from May 2016 to June 2017). The patients in the experimental group and the control group underwent periodontal examination, and the baseline was recorded. The control group was treated with routine treatment, the patients treated on the basis of analysis of Periocline ointment in the treatment of two groups of patients treated for 4 weeks. Results After treatment, the patients in the experimental group for attachment level (4.84±0.74) mm, probing depth (3.45±0.47) mm, bleeding index for (2.43±0.52) was significantly better than the control group related clinical indicators of periodontal probing depth and attachment level, with statistical significance (P<0.05). The number of effective cases in the experimental group was 47 cases (94%), significantly higher than the control group, the number of effective treatment in 44 cases (88%), the difference was statistically significant (P<0.05). Conclusion Periocline ointment as the auxiliary treatment of chronic periodontitis can improve the treatment efficiency, improve clinical indexes of patients, is worthy of clinical application.
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Objective To study the clinical effect of Periocline ointment as the auxiliary treatment of chronic periodontitis. Methods 100 cases of chronic periodontitis in our hospital were selected as the subjects (from May 2016 to June 2017). The patients in the experimental group and the control group underwent periodontal examination, and the baseline was recorded. The control group was treated with routine treatment, the patients treated on the basis of analysis of Periocline ointment in the treatment of two groups of patients treated for 4 weeks. Results After treatment, the patients in the experimental group for attachment level (4.84±0.74) mm, probing depth (3.45±0.47) mm, bleeding index for (2.43±0.52) was significantly better than the control group related clinical indicators of periodontal probing depth and attachment level, with statistical significance (P<0.05). The number of effective cases in the experimental group was 47 cases (94%), significantly higher than the control group, the number of effective treatment in 44 cases (88%), the difference was statistically significant (P<0.05). Conclusion Periocline ointment as the auxiliary treatment of chronic periodontitis can improve the treatment efficiency, improve clinical indexes of patients, is worthy of clinical application.
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<p><b>OBJECTIVE</b>To explore clinical efficacy of Yiguanjian Decoction (YD) combined Adefovir Dipivoxil Tablet (ADT) in treating HBeAg negative chronic viral hepatitis B (CVHB) active compensated liver cirrhosis (LC) patients.</p><p><b>METHODS</b>Totally 68 HBeAg negative CVHB active compensated LC patients initially treated were assigned to the treatment group and the control group using random digit table, 34 in each group. Patients in the control group took ADT alone, 10 mg each time, once per day. Those in the treatment group additionally took YD, one dose per day. The therapeutic course for all was 48 weeks. Levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBil) were detected once in every two weeks. Hepatitis B virus (HBV)-DNA and four items of serum liver fibrosis [procollagen type I (PCN), hyaluronidase (HA), procollagen III peptide (PCIII), laminin (LN)] were detected once per every 4 weeks. Abdominal ultrasound B was performed before and after treatment. The inner diameter of the portal vein and the size of spleen were recorded. The fibrosis degree of liver was evaluated using Fibroscan. Efficacy of Chinese medicine (CM) was evaluated between the two groups before and after treatment using CM syndrome integrals. Efficacy of Western medicine (WM) was also evaluated between the two groups using Child-Pugh grading. Results Compared with before treatment in the same group, ALT and AST levels restored to normal levels, HBV-DNA turned negative (HBV-DNA < or = 1 x 10(2)) in the two groups after 48-week treatment. Besides, levels of TBil, ALB, PCIV, HA, PCIII, and LN obviously decreased (P < 0.05, P < 0.01). Results of ultrasound B showed the inner diameter of the portal vein and the size of spleen decreased. Fibroscan results showed that the elasticity value of the liver obviously decreased (P < 0.05). Besides, post-treatment levels of PCIV, HA, PCEJ, and LN, and the elasticity value of the liver decreased more obviously in the treatment group than in the control group (P < 0.01). There was no statistical difference in post-treatment levels of ALT, AST, TBil, ALB, inner diameter of the portal vein, or the size of spleen between the two groups (P > 0.05). Compared with before treatment in the same group, scores of Chinese medical syndrome and Child-Pugh scores decreased in the two groups after treatment (P < 0.05, P < 0.01). Besides, scores of Chinese medical syndrome decreased more obviously in the treatment group than in the control group (P < 0.05). The effective rate was 8824% (30/34) in the treatment group, higher than that of the control group [67.65% (23/34)] with statistical difference (P <0.05). Conclusion Combined treatment of YD and ADT could significantly improve symptoms of CM and fibrosis degree of liver of HBeAg negative CVHB active compensated LC patients.</p>
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Humans , Adenine , Therapeutic Uses , Alanine Transaminase , Blood , Antiviral Agents , Therapeutic Uses , Aspartate Aminotransferases , Blood , Bilirubin , Blood , DNA, Viral , Blood , Drugs, Chinese Herbal , Therapeutic Uses , Hepatitis B e Antigens , Blood , Hepatitis B, Chronic , Drug Therapy , Liver Cirrhosis , Drug Therapy , Virology , Organophosphonates , Therapeutic Uses , TabletsABSTRACT
Background and purpose:P38 mitogen-activated protein kinase (P38MAPK) signal transduction pathway is involved in occurrence, development and transfer process in a wide variety of tumors. Urokinase-type plasminogen activator (uPA) plays an important role in tumor invasion and metastasis. This study aimed to explore the clinical signiifcance of the P38MAPK signaling pathway and the expression of uPA in ovarian cancer.Methods:The expressions of uPA, P38MAPK, ERK and AKT were detected in 49 cases of cervical adenocarcinoma by immunohistochemistry. The expressions of uPA and P38MAPK were detected by Western blot in ovarian cancer cell lines HO8910, HO-8910PM, SKOV3 and CAOV3. The changes of uPA and P38MAPK were detected by SB203580, a speciifc inhibitor of P38MAPK signal transduction pathway.Results:The result of immunohistochemical method showed that positive expression rates for uPA, P38MAPK, ERK and AKT were 61.22%, 57.14%, 53.06% and 55.10%, respectively. The expression of the uPA was positively correlated with the P38MAPK (r=0.865,P=0.001), and related with clinicopathologic stage, differentiated degree, lymph node metastasis, but not related with age and histologic type (P>0.05). The expressions of AKT and ERK were related with the lymph node metastasis and greater omentum metastasis(P0.05). The expression of uPA in HO-8910PM was higher than that in ovarian cancer cell lines HO8910, SKOV3 and CAOV3, and the expression of uPA reduced when the P38MAPK signal transduction pathway was cut off by the SB203580. The expressions of P38MAPK and uPA were negatively correlated with the prognosis of ovarian cancer (Log-rank=3.897 and 11.044,P=0.048 and 0.001). Conclusion:The P38MAPK signal transduction pathway was activated in ovarian cancer. The activated P38MAPK signal transduction pathway can raise the expression of uPA, which may contribute to the development of ovarian cancer. The result indicates that the P38MAPK signal transduction pathway and uPA might play an important role in the invasion and metastasis of ovarian cancer. P38MAPK and uPA might be useful markers for evaluating prognosis of ovarian cancer.
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OBJECTIVE: To observe the blood-brain barrier permeability of scutellarin ethyl ester, the structural modification product of scutellarin, and to explore the mechanism of nerve protection on cerebral ischemia. METHODS: By co-culturing brain microvascular endothelial cells (BMVEC) and astrocytes cells (AC) in vitro, blood-brain barrier model was established. The active components were analyzed by HPLC. Rat cerebral cortical neurons cells were cultivated in vitro, and the survival rate was determined by MTT assay. The model of rat cortical neurons cell injury was established by glutamate and KCl, and the change of fluorescence intensity was detected by Laser Scanning Confocal Microscopy (LSCM). RESULTS: Compared with control group, scutellarin ethyl ester could penetrate BBB, promote the survival of cortex nerve cells in vitro significantly(P < 0.01), and inhibit intracellular calcium overload induced by 500 μmol · L-1 glutamate (P < 0.01). CONCLUSION: Scutellarin ethyl ester can pass through BBB and has neuroprotective effect by regulation of calcium signal pathway to resist cerebral ischemia. Copyright 2012 by the Chinese Pharmaceutical Association.
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<p><b>OBJECTIVE</b>To compare the effectiveness and side effects of two chemotherapy regimens [pirarubicin + cytarabine (TA) and daunorubicin + cytarabine (DA)] in patients with acute myeloid leukemia (AML).</p><p><b>METHODS</b>From Oct 2006 to Jul 2009, there were 207 newly diagnosed AML patients randomized into DA or TA group from 72 centers all over the country. The aim of this clinical trial is to observe and evaluate complete remission rate (CR), total remission rate (TRR), and side effect after one or two circles of therapy.</p><p><b>RESULTS</b>In 198 evaluable patients, 126 cases in TA group and 72 in DA group were evalvable, with a ratio of 1.75:1. CR was 69.8% and TRR (CR + PR) was 81.8% in TA group and 63.9%, 80.9% in DA group, correspondingly (P > 0.05). For patients with subtype M(2), CR (77.1%) in TA group was higher than that in DA (60%). There was no difference in side effect between the two groups.</p><p><b>CONCLUSION</b>There is no difference of the effect between TA and DA chemotherapy for newly diagnosed AML patients. But for subtype M(2), TA is more efficacy. And there is no difference in side effect between the two regimens.</p>
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Humans , Antineoplastic Combined Chemotherapy Protocols , Therapeutic Uses , Cytarabine , Daunorubicin , Leukemia, Myeloid, Acute , Drug Therapy , Prospective StudiesABSTRACT
<p><b>OBJECTIVE</b>To observe the pharmacodynamics of Jingjielianqiao decoction in promoting the healing of acute radiation-induced skin ulcers in mice.</p><p><b>METHODS</b>Skin ulcers were induced in mice by radiation, and Jingjielianqiao decoction, rivanol or normal saline was applied for the treatment. The swelling of the legs was measured in mice using volumetric method, and the ulcer area after treatment was compared among the 3 groups.</p><p><b>RESULTS</b>No significant difference was found in the anti-inflammatory effects between Jingjielianqiao decoction group and rivanol group (P>0.05), but a significant difference was noted between Jingjielianqiao decoction group and normal saline group and also between rivanol group and normal saline group (P<0.01). Significant differences were observed in the ulcer healing among the 3 groups (P<0.01).</p><p><b>CONCLUSION</b>Jingjielianqiao decoction can significantly suppress radiation-induced acute inflammation and shows better effect than rivanol in promoting the healing of acute radiation-induced skin ulcer in rats.</p>
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Animals , Female , Rats , Drugs, Chinese Herbal , Pharmacology , Therapeutic Uses , Phytotherapy , Radiodermatitis , Drug Therapy , Rats, Wistar , Skin Ulcer , Drug Therapy , Wound HealingABSTRACT
<p><b>OBJECTIVE</b>To investigate the application of surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI) protein chip in diagnosis and Chinese medicie syndrome type researching of systemic lupus erythematosus (SLE).</p><p><b>METHODS</b>Eighteen female SLE patients of mild/moderate degree with yin-deficiency caused internal heat syndrome (YDHS) were enrolled in the treatment group, and 15 women healthy volunteers was set up as the control group. Using SELDI method, the pre-, mid- and post-treatment peripheral blood mononuclear cell (PBMC) protein fingerprint of them was created respectively, which was then managed to screen out the markers by using ZUCI-PDAS package for establishing a diagnosis model.</p><p><b>RESULTS</b>Study was completed in 15 cases of the treatment group with 2 cases dropped out and 1 case lost. Before treatment, 44 protein peaks in the treatment group were found significantly different to those in the control group (P<0.01), and the sensitivity and specificity of the created models, 10542 Da m/z and 2554 Da m/z, reached 100%. After a 12-week treatment, 30 peaks were found significantly different between the two groups (P<0.01), and the sensitivity of 3365, 7104, 3882 and 6796 Da m/z created peak models was 100%, its specificity being 93.33%. Comparing the 35 samples (pre-, mid- and post-treatment) got from the treatment group with the 15 samples from the control group, significant difference was found in 55 peaks (P<0.01), the sensitivity and specificity of the 7103, 3882, 7143 Da m/z created peak models was 100% and 91.43% respectively.</p><p><b>CONCLUSION</b>Significant differences of PBMC protein expression patterns were found between SLE patients of YDHS and healthy persons at times of before, during and after treatment, suggesting that SELDI may be used as a new method to create the diagnosis model, and its application in effecter protein screening, activity scoring and Chinese medicine syndrome type researching are expectable and waiting for further study in depth.</p>
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Adolescent , Adult , Female , Humans , Middle Aged , Young Adult , Case-Control Studies , Leukocytes, Mononuclear , Metabolism , Lupus Erythematosus, Systemic , Diagnosis , Protein Array Analysis , Sensitivity and Specificity , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Methods , Yin DeficiencyABSTRACT
This study was aimed to investigate the influences of interferonalpha (IFN-alpha) on expressions of CCR7, interleukin10 (IL-10) and IL-12p70 in dendritic cells (DCs) from patients with chronic myeloid leukemia (CML). In addition to stem cell factor (SCF), granulocyte-macrophage colony stimulating factor (GM-CSF), tumor necrosis factor-alpha (TNF-alpha) and IL-4, IFN-alpha was added to the serum-free medium of DCs. After culture for 10-14 days, phenotypes and function of CML-DCs were evaluated respectively by flow cytometry and methyl thiazolyl tetrazolium (MTT) assay. Chromosome of DCs was analyzed by displaying G banding assay. The concentrations of IL-10 and IL-12P70 in supernatants were evaluated by enzyme-linked immunosorbent assay (ELISA). The results showed that the expressions of CD40, CD83, CD86 and CCR7 and the OD value in allogeneic mixed-lymphocyte reaction (MLR) in group with IFN-alpha (300 U/ml) were twice as high as those in group without IFN-alpha. The percentage of Ph1 positive cells and concentrations of IL-10 and IL-12 P70 were reduced in group with IFN-alpha. It is concluded that the defective phenotypes and functions of CML-DCs can be recruited partly by IFN-alpha. The mechanism may lie in the facts that expression of CCR7 and co-stimulatory molecules is promoted and the inhibitory effect of IL-10 on CML-DCs is relieved partly through the regulation of IFN-alpha.
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Humans , Cells, Cultured , Dendritic Cells , Cell Biology , Interferon-alpha , Pharmacology , Interleukin-10 , Genetics , Metabolism , Leukemia, Myelogenous, Chronic, BCR-ABL Positive , Genetics , Allergy and Immunology , Philadelphia Chromosome , Receptors, CCR7 , Genetics , MetabolismABSTRACT
The study was aimed to investigate the influence of interferon alpha (IFN-alpha) on the expressions of Fas and Fas ligand (FasL) in dendritic cells (DCs) from patients with chronic myeloid leukemia (CML). In addition to adding stem cell factor (SCF), granulocyte-macrophage colony-stimulating factor (GM-CSF), tumor necrosis factor alpha (TNF-alpha) and interleukin 4 (IL-4), the IFN-alpha was added to the serum-free medium for DCs. After culturing for 10 - 14 days, cell phenotype and percentage of Ph(1) chromosome were detected by different methods. The expression of Fas or FasL on CML-DCs and cell cycle of DCs labeled with propidium iodine (PI) were measured by flow cytometry. The concentration of sFas in supernatants was analyzed by enzyme-linked immunosorbent assay (ELISA). The results indicated that the expression of co-stimulatory molecules were improved significantly while the percentages of Ph(1) positive cells decreased. The level of Fas on cells was up-regulated and the concentration of sFas decreased. However, the expression of FasL was negative. The ratio of apoptosis rose gradually while the concentration of IFN-alpha increased. It is concluded that IFN-alpha can accelerate the apoptosis of Ph(1) positive cells through Fas/FasL pathway, so the number of Ph(1) negative cells increases relatively.